Saracens will host Harlequins in the Aviva Premiership at Wembley Stadium for the third time.
The Men in Black will play host to Quins on March 28 2015, with the game being held at the national stadium.
Sarries last played Quins at Wembley in March 2014 in front of a world record crowd of 84,000 fans, with Mark McCall's side running out 39-17 winners.
In 2012, Sarries took on Quins at Wembley for the first time, losing 24-19 in front of a marginally smaller crowd.
Singer Pixie Lott will perform live as Saracens bid to host another successful afternoon of entertainment and rubgy.
Chairman, Nigel Wray, told the club website: “Saracens playing Harlequins at Wembley has become a calendar event on the sporting calendar, and we look forward to another wonderful occasion.”
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